Ion AmpliSeq™ Cancer Hotspot Panel v2

Catalog number:  4475346

Description:

The Ion AmpliSeq™Cancer Hotspot Panel v2 is a single pool of primers used to perform multiplex PCR for preparation of amplicon libraries from genomic "hot spot" regions that are frequently mutated in human cancer genes (download the list of targeted genes and mutationsfor the Ion AmpliSeq™Cancer Hotspot Panel v2). Building on the mutations included in our original Ion AmpliSeq™Cancer Panel, the latest Ion AmpliSeq™ready-to-use panel provides:

• Maintained compatibility with FFPE samples while expanding mutational content for broader coverage of additional genes and "hot spot" mutations
• Extremely uniform coverage for more efficient sequencing and cost savings
• Enhanced primer design with optimization of new primer sets and even lower strand bias for added confidence in accurate variant calling
• Improved variant detection with Torrent Suite Software v3.0 and Variant Caller Plugin in low allele variant detection along with improved indel sensitivity

Simplicity, Speed, and Scalability of Ion AmpliSeq™Technology
Sustaining the simplicity of the Ion AmpliSeq™technology, the Ion AmpliSeq™Cancer Hotspot Panel v2 enables cancer genetic studies from FFPE tissues with as little as 10 ng of input DNA for targeted library construction. The Ion AmpliSeq™Cancer Hotspot Panel v2 also maintains the revolutionary workflow using standard PCR equipment and a simple PCR reaction for ultra-high multiplex PCR-based target selection, with no need for extensive capital investment. Additionally, the Ion AmpliSeq™Cancer Hotspot Panel v2 continues to enable you to achieve the fastest time to targeted libraries in approximately 3.5 hours. Scalability and flexibility is also preserved with the Ion AmpliSeq™Cancer Hotspot Panel v2, allowing the ability to sequence one sample or multiplex barcoded samples using any of the Ion chips.

Improved Variant Detection with Torrent Suite Software v3.0
The intuitive Torrent Suite Software and Variant Caller Plugin enable you to go from extracted DNA to variant calls in as little as 10 hours. With improvements to algorithms, you will see more sensitive indel detection along with more sensitive SNP detection with lower frequency alleles. Ion Reporter™Software can then be used for annotation of variants in routine DNA assays for the advancement of clinical research.